UNDERSTANDING THE MECHANISM OF SILVER NANOPARTICLE TOXICITY

GARCÉS M ; MARCHINI T; GALDOPORPORA J; ZUBILLAGA M; ALVAREZ S; MAGNANI N; PECORELLI A; VICO T; MORETTON M; VALACCHI G; CALABRÓ V; CACERES, LOURDES CATALINA; SALGUEIRO J; DESIMONE M; EVELSON P

VIRTUAL

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIAS SAIC-SAI-SAFIS 2020; 2020

Sociedad Argentina de Investigación Clínica (SAIC)

Nanotoxicology has increasingly gained attention along with theAgNP applications development. As inhalation is one of the mainroutes of exposure, the aim of this study was to evaluate the AgNPharmful effects in the lung. Characterization of AgNP was performedby DLS, TEM and EPR, while biodistribution studies were done usingradio labeled 99mTc-AgNP. In vivo studies in balb/c mice intranasallyinstilled with 0.1 mg AgNP/kg b.w were performed, where O2metabolism was analyzed 1h after AgNP inhalation. In vitro studiesusing A549 cells and a 3D lung tissue model, were done after 1hexposure to 2.5 μg AgNP/mL. AgNP showed a hydrodynamic diameterof 17 ± 6 nm and were able to initiate the hemolytic cleavage ofH2O2 leading to OH? production. In vivo results showed the lung asthe main deposition organ, where AgNP initiate alveolar epitheliumbarrier injury, revealed by increased protein content and total cellcount in BAL samples (46%;120%; p