Lens as a novel target of urban air pollutants: evaluation of the redox balance

HVOZDA ARANA, AILÉN; MARTINEFSKI, MANUELA; LLESUY, SUSANA; FERREIRA, SANDRA; MARCHINI, TIMOTEO; REIDES, CLAUDIA; BERRA, ALEJANDRO; LASAGNI VITAR, ROMINA; TAU, JULIA; TRIPODI, VALERIA; EVELSON, PABLO

N/A (cancelado por pandemia COVID-19)

Congreso; ARVO Annual Meeting; 2020

Association for Research in Vision and Ophthalmology

Purpose : Particulate matter (PM) present in air pollution induces adverse effects on the eye. Oxidative stress has been suggested to play a key role in the ocular surface response triggered by urban air pollutants. It is well-known that lens antioxidant system maintains the redox status of nearby ocular structures. The aim of the study was to evaluate the redox balance in mice lens after the exposure to urban air pollution.Methods : 8-week-old Balb/c male mice were exposed to urban air or filtered air (UA-group and FA-group, respectively) in exposure chambers located in highly populated area of Buenos Aires city (average level of PM: 25.6 ± 0.8 μg/m3). The animals were exposed for 8 h/day, 5 days/week, up to 12 weeks. The local committee for animal care (CICUAL-FFYB, CUDAP 50946/16) approved the experimental model. Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), Glutathione S-transferase (GST), and Thioredoxin reductase (TrxR) activities, levels of reduced and oxidized glutathione (GSH and GSSG) and protein oxidation (PO) were evaluated in lens lysates.Results : After 1 and 2 weeks of exposure, UA-group presented no significant differences in all measurements compared to the FA-group, except for SOD activity that was increased after 1 week (107%, p