Preliminary effect of the administration of E. coli lipopolysaccharide (LPS) on the ascorbyl radical (A?) content in brain from rats overloaded with Fe.

PILONI, N.E.; ROUSSEAU, I; PUNTARULO, S.

Buenos Aires

Congreso; Reunión Anual de Biociencias: SAIC, SAFE, SAB, SAP, NANOMEDar, AACyTAL; 2020

SAIC

The aim of this study was to evaluate the effect of LPS in brains from rats treated with acute Fe-dextran. Sprague Dawley rats were intraperitoneally injected with a single dose of Fe-dextran (500 mg/kg) and LPS (4 mg/kg). The nitric oxide (NO) content was determined in blood by Electronic Paramagnetic Resonance Spectroscopy (EPR), with hemoglobin at 77K. The administration of LPS significantly increased (p≤0.01, ANOVA) the NO content in the blood of the control animals (1.9 ± 0.1 AU), in the presence of either Fe-dextran, or LPS or LPS+Fe-dextran (1.5-, 10.5- and 14.2-folds, respectively). After 6 h of treatment, the administration of Fe-dextran or Fe-dextran+LPS, showed a significant increase of the Fe content in total brain, determined by acid mineralization, in the absence (20-fold), and in presence of LPS (17-fold). The labile Fe pool (LIP) content, determined using calcein, was increased 6 h after Fe administration, and returned to control values after 8 h. The content of A?, determined by EPR, significantly increased after 6 h, in animals overloaded with Fe-dextran in the absence of LPS (41%, p