Optimal conditions for the treatment of samples for the storage of red blood cells stored in a blood bank

LAIRION F; GARCÍA, M; REPETTO, MG; VELLICE, A

Congreso; Reunión Anual de Sociedades de Biociencia. SAIC, SAI, SAFIS; 2020

Sociedad Argentina de Investigación Clínica

Red blood cell (RBC) storage injury is the deterioration caused during its conservation in the blood bank. One of its causes is the autoxidation of hemoglobin (Hb), which accumulates peroxides (XOOH), oxidized Hb species (EOHb) and fluorescent degradation products (FHP). The bibliography presents different protocols for the same method. The objective was to define the optimal conditions to evaluate EOHb, FHP and XOOH in 10 units of GR stored in the blood bank (DRG). The EOHb were evaluated with a spectral sweep between 500 and 700 nm at different concentrations and pH. The most influential factor was the Hb concentration of the sample, adjusted to 0.7 mg/mL, followed by the pH, adjusted to 7.5. The FHPs represent the intracellular content of H2O2 as a fluorescence peak at 485 nm under excitation at 321 nm. The emission spectrum between 400 and 600 nm was analyzed at different concentrations and storage conditions. A peak was not observed at 485 nm, but the emission average between 470 and 490 nm was correlated with it and was more precise (CV 5.5% vs 11.4%, p