Cellular Fe in Fetal brain gamma-irradiated both in vivo and in vitro.

ROBELLO E,; PILONI NE,; PUNTARULO S.

Salta, Argentina

Congreso; XXXIX Annual Meeting of Argentinean Biophysical Society SAB 2010; 2010

SAB

Cellular Fe in fetal brain gamma-irradiated both in vivo and in vitro Robello Elizabeth, Piloni Natacha, Puntarulo, Susana.   Physical Chemistry-PRALIB, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956, Buenos Aires, Argentine e-mail: npiloni@ffyb.uba.ar   This study was aimed to test comparatively the effect of g-irradiation on Fe content in the fetal brain irradiated in vitro and in vivo. In vivo irradiation was performed by exposure of the mother to g-irradiation at 17th gestational days (GD) and cells of rat brains were kept on culture for 0, 1, 2 and 4 h post-irradiation (pi). In vitro irradiation was performed exposing isolated fetal brain cells taking from foetuses after 17 GD. In the in vivo model there was a significant increase in the total Fe content, assessed spectrophotometrically, at 4 h pi (4.2±0.1, 5.2±0.3, 5.1±0.8 and 14±1 nmol/106 cells for 0, 1, 2 and 4 h pi). A significant reduction on the labile iron pool (LIP), assessed employing calcein, at 2 h pi (0.27±0.04 nmol/106 cells), was detected as compared to non-irradiated cells (0.39±0.02 nmol/106 cells). In the in vitro model, there were neither alterations on the total Fe content (3.2±0.7, 3.3±0.9, 4.0±0.2 and 3.5±0.3 nmol/106 cells at 0, 1, 2 y 4 h pi) nor on the LIP (0.42±0.03, 0.41±0.02, 0.4±0.1 and 0.51±0.03 nmol/106 cells at 0, 1, 2 y 4 h pi). These results suggest that alterations on Fe content in fetal brain cells depend on the complex interactions between the maternal and fetal plasma that can not be reproduced adequately by the in vitro model. Supported by grants from UBA and CONICET.