Arterial hypertension induced by saline overload: role of chloride anion

PRINCE PD; GALLEANO M; ROBBESAUL GD; PANDOLFO M; KOUYOUMDZIAN NM; KIM G; CHOI MR

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC) ? Reunión conjunta SAFE ? SAB ? SAP 2019; 2019

SAIC-SAFE-SAC-SAP

A chronic saline (as sodium chloride) overload (SO) in the diet induces a renal inflammatory response and oxidative stress, which lead to the development of hypertension. The aim of this work was to demonstrate the hypothesis that chloride anion (Cl-), besides sodium cation (Na+), is involved in these inflammatory and oxidative responses. These alterations might be diminished if Cl- is replaced by other anion (like citrate), or if Na+ is replaced by other cations.Male Wistar rats were randomly divided into four experimental groups (n=8): control (C); SO (NaCl 8% W/W); high Na+ without Cl- (Na: Na3C6H5O7 11,8%); high Cl- without Na+ (Cl: CaCl2 3,80%; KCl 3,06% and MgCl2 1,30%). After three weeks, systolic blood pressure (SBP) was measured, and rats were housed in metabolic cages in order to collect 24-hour urine to assess renal function. Oxidative stress parameters were measured in renal cortex: TBARS production andantioxidant enzymes activities and expression:superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). In all the experimental groups we observed a significant increase of diuresis (*p