Brain mitochondrial function in glaucoma

HVOZDA ARANA, AILÉN; CALABRÓ, VALERIA; LERNER, FABIÁN; JANEZIC, NATASHA; REIDES, CLAUDIA; FERREIRA, SANDRA; LASAGNI VITAR, ROMINA; MARCHINI, TIMOTEO; EVELSON, PABLO

Mar del Plata

Congreso; LXIV Reunión Anual de la SAIC; 2019

Sociedad Argentina de Investigación Clínica

Glaucoma is the second leading cause for blindness worldwide and damages structures in the brain, including the primary visual cortex. The aim was to evaluate the alterations of mitochondrial function in an experimental glaucoma model. Three-month female Wistar rats were divided in two groups (n= 8): glaucoma (GG) in which rats were operated under a microscope by cauterized two of the episcleral veins and control (CG) which received a sham procedure. Seven days after surgery rats were euthanized, brain cortex was separated, and mitochondria were isolated (CICUAL FFyB n° 3314). The following markers were evaluated: oxygen consumption (OC), ATP production, hydrogen peroxide (H2O2) and superoxide anion (O2-) production, the activities of superoxide dismutase (SOD), complex I-III and complex II-III, cardiolipin content, mitochondrial membrane potential, and NADPH oxidase-4 (NOX4) expression. Comparing to CG, GG showed a decrease in ATP production (23 %, p