IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Inhibition of alpha-glucosidase and pancreatic lipase by a plant extract of Drymis andina: in vitro an din vivo studies
Autor/es:
LIZARRAGA E; FRAGA CG; GASTALDI B; GORZALCZANY SB; MOSSE JI; GONZÁLEZ SB; GALLEANO M
Lugar:
Mar del Plata
Reunión:
Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019
Institución organizadora:
SAIC
Resumen:
There is a renewed momentum backed by the WHO to discover newer, cheaper and better anti-obesity agents derived from plants. In this work we analyzed an aqueous extract from a native plant, Drymis andina. as inhibitor of digestive enzymes, which is a promising mechanism of action on this topic.Aqueous extract (5%) of D. andina was investigated in terms of phenolics compounds, flavonoids, coumarins, alkaloids, saponins, tannins, mucilage, sugars and proteins. In vitro lipase and alpha-glucosidase inhibition was measured by colorimetric methods. Acute toxicity studies and in vivo inhibition of enzymes assays were carried out on C57BL6 mice. Acarbose and orlistat were used as positive controls.The lyophilized extract of D. andina mainly showed a high phenolic compounds content (total phenol content 302±1 mg/g, total flavonoid content 45.1±0.5 mg/g, hydroxycinnamic acids 174±17 mg/g, o-dihydroxy-phenols 71±1 mg/g). In the in vitro assays, the IC50 were 0.10 ± 0.03 mg/mL and 2.04 ± 0.08 mg/mL for alpha-glucosidase and lipase, respectively. No toxic effect was evident for the extract in the acute toxicity study by using the dose of 2 g/kg body weight. For oral maltose tolerance test, doses of 45, 90, and 180 mg/kg decreased significantly the postprandial glycemia in 34%, 38% and 68%, respectively. For oral lipid tolerance test, dose of 180 mg/kg decreased significantly the postprandial triglyceridemia in 78%.In summary, the aqueous extract of D. andina did not show acute toxicity and was effective as inhibitor of alpha-glucosidase and lipase both in vitro and in vivo. The introduction of D. andina as infusion before the consumption of refined carbohydrates and/or fat rich food could be beneficial not only to prevent fat deposition but also to reduce the negative impact on metabolism promoted by high levels of postprandial glucose and triglycerides.