LENS REDOX IMBALANCE AFTER URBAN AIR POLLUTION EXPOSURE

LASAGNI VITAR, ROMINA M.; MARTINEFSKI MANUELA; TRIPODI, VALERIA P.; LLESUY, SUSANA F.; JANEZIC, NATASHA S.; MARCHINI, TIMOTEO; REIDES, CLAUDIA G.; BERRA, ALEJANDRO; HVOZDA ARANA, AILEN G.; TAU, JULIA; EVELSON, PABLO A.; FERREIRA, SANDRA M.

Mar del Plata

Congreso; Reunión Anual de Biociencia 2019 - SAIC-SAFE-SAB-SAP-NANOMEDar-AACyTAL-HSC; 2019

Particulate matter (PM) present in air pollution produces adverse effects on the eye. Oxidative stress has been suggested to play a key role in the toxic mechanism. Lens antioxidant system maintains the redox status of nearby ocular structures. The aim of the study was to evaluate the redox balance in mice lens after the exposure to urban air pollution. 8- week-old Balb/c male mice were exposed to urban air or filtered air (UA-group and FA-group, respectively) in exposure chambers located in highly populated area of Buenos Aires city (averagelevel of PM: 25.6 ± 0.8 μg/m3). The animals were exposed for 8 h/day, 5 days/week, up to 12 weeks (CICUAL-FFYB, CUDAP 50946/16). Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) activity, levels of reduced and oxidized glutathione (GSH and GSSG) and protein oxidation (PO)were evaluated in lens lysates. After 1 and 2 weeks of exposure, UA-group presented no significant differences in all measurements compared to the FA-group, except for SOD activity that was increased after 1 week (107 %, p