The expression of NOX in renal cortex of L-NAME treated rats. Effects of (?)-epicatechin

GALLEANO M; FRAGA, CG; PRINCE, PD

Ferrara

Congreso; SFRRE 2019 Redox Homeostasis: From Signaling to Damage; 2019

SFRRE

The expression of NOX in renal cortex of L-NAME treated rats. Effects of (?)-epicatechinThis work evaluated the effects of dietary (?)-epicatechin (EC) on renal functions of rats receiving L-NAME for 4 d, in terms of parameters of kidney function and oxidative stress, including the expression of NOX1, NOX2 and NOX4 subunits in kidney cortex. Experimental groups were: control (C): control diet; L-NAME (L): control diet+ drinking water added with L-NAME (360 mg/l); L-NAME+EC (LE): diet with EC (4g/kg diet) + L-NAME solution. Plasma urea and creatinine were significantly higher in L respect to C and LE indicating and incipient kidney failure. At renal level, MDA and protein nitrotyrosine levels were higher in L respect to C and LE groups. L group showed increased levels of NOX2 subunits (+44% in gp91phox and +59% in p47phox), of NOX4 subunit (+33%), and of the NOXO1 subunit (+50%) respect to C group; LE group did not show these increases. In summary, L-NAME induced alterations in renal function that were attenuated by EC in association with the modulation of the expression of NOX subunits. Further experiments should be necessary to understand the mechanism of L-NAME-induced NOX expressions.Support: UBACyT 20020160100132BA (CGF) and 20020170100586BA (MG) and PIP 11220170100585CO (MG).