IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Protective Effects of the Synthetic Cannabinoids CP55,940 and JWH-015 on Rat Brain Mitochondria upon Paraquat Exposure.
Autor/es:
BUSTAMANTE, J.; VELEZ-PARDO, C.; JIMENEZ-DEL RÍO, M.; LORES-ARNAIZ, S.
Lugar:
Free Radicals and Antioxidants in Chile, VI Meeting of the SFRBM South American Group
Reunión:
Congreso; Free Radicals and Antioxidants in Chile, VI Meeting of the SFRBM South American Group; 2009
Institución organizadora:
SFRBM South American Group
Resumen:
The effect of cannabinoids CP55,940 and JWH-015 in rat brain mitochondria after acute paraquat (PQ) exposure was analysed by their ability to scavenge superoxide anion radicals and hydrogen peroxide in either sub-mitochondrial particles or intact mitochondria treated with PQ. Oxygen consumption and mitochondrial membrane potential (DYm) were determined as parameters of mitochondrial function in the presence of PQ alone or in combination with the cannabinoid agonists. Both cannabinoids effectively attenuate mitochondrial damage against PQ-induced oxidative stress by scavenging superoxide anion radical and hydrogen peroxide. While H2O2  generation increased almost 3-fold upon PQ treatment (p<0.01) compared to untreated mitochondria, CP55,940 and JWH-015 significantly reduced mitochondrial H2O2 by 44% and 50%, respectively when co-incubated with PQ. Both cannabinoids were not able to rescue mitochondrial respiration from PQ treatment, effects on state 4 and 3 respiration, will be discussed. Mitochondria incubated with 250 mM PQ,  show  moderate loss of basal Dym by about 22% compared to untreated mitochondria. Interestingly, both CP55,940  and JWH-015 were able to return Dym to basal state levels when co-incubated with PQ. In summary, CP55,940 and JWH-015 protect mitochondria against PQ-induced oxidative stress by maintaining Dym and efficiently scavenging O2.- and H2O2. Understanding the mechanistic action of cannabinoids on mitochondria might provide insights into more effective therapeutic approaches for Parkinson’s disease.