Remote ischemic pre and postconditioning: simila degree of cardioprotection but different mechanism

BIN EP; GELPI RJ; BUCHHOLZ B; PÁEZ DT; DONATO M

Buenos Aires

Congreso; IV International Congress in Translational Medicine. International Master Program in Biomedical Sciences; 2018

Background: It is known that brief episode(s) of ischemia/reperfusion applied prior or after myocardial ischemia to peripheral tissue located at a distance from the heart significantly reduce myocardial infarct size. This phenomenon is called remote ischemic conditioning (RIc).The aim was compared the efficacy of RIc in protecting the heart when the RIc stimulus is applied prior to myocardial ischemia (remote ischemic preconditioning; rIPC), or at the onset of myocardial reperfusion (remote ischemic postconditioning; rIPost). Methods: In order to induce myocardial infarction, we used mice (FVB, 24 g) which were subjected to 30 min of left coronary artery occlusion followed by 120 min of reperfusion. rIPC and rIPost were induced by 3 cycles of 5min of ischemia followed by 5 min of reperfusion of the left femoral artery. The infarct size was measured using Evans blue (Risk Area) and Triphenyl-tetrazolium chloride (Infarctsize).Results:The ischemia/reperfusion protocol (30 min of ischemia and 120 min of reperfusion) induced an infarct size of 69.13±3.22 %. The rIPC and rIPost significantly reduced infarct size to 51.17±4.36 (p