IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MECHANISMS OF FIRST AND SECOND WINDOW OF PROTECTION OF PRE-ISCHEMIC VAGAL STIMULATION ON MYOCARDIAL INFARCTION IN MICE
Autor/es:
VERENA FRANCO RIVEROS; MARÍA CARLA GRECO; NAHUEL MÉNDEZ DIODATI; VERÓNICA CASANOVA; RICARDO J. GELPI; JAZMÍN KELLY; EDUARDO A. BERNATENÉ; BRUNO BUCHHOLZ
Lugar:
CABA
Reunión:
Congreso; IV INTERNATIONAL CONGRESS IN TRANSLATIONAL MEDICINE; 2018
Resumen:
We have previously proven that vagal stimulation (VS) decreases infarct size (IS) when applied before ischemia, thus mimicking classic preconditioning. Our objective was to study the chronology and mechanisms of the protective effect of VS, and to assess whether the protection is comprised of a first and second window. Mice were randomly assigned to different protocols (n=5-7 per group). The control group had 30 min of regional myocardial ischemia and 2 h of reperfusion (I/R). The other groups consisted of 10 min of pre-ischemic VS (pVS) and different times of recovery between VS and I/R protocol: 5 min, 3-6-12-24-48-72-96 h. The 5 min, 3 h and 72 h groups were redone to administer a muscarinic receptor inhibitor (atropine). pVS-5min and pVS-72h were given a mitochondrial K+ ATP channel blocker (5-HD) and a nitric oxide synthase inhibitor (L-NAME). All animals were catheterized to measure ventricular function. Hearts were dyed with Evans Blue and incubated in TTC to measure IS. pVS-5min had smaller IS in comparison to I/R (44±3% and 57±2%, respectively; p