IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Overexpression of Thioredoxin in heart has a protective effect after exposure to ambient air particles
Autor/es:
GARCÉS, MARIANA; TASAT, DEBORAH; ALVAREZ, SILVIA; GUAGLIANONE, ALEJANDRO; CALABRO, VALERIA; GELPI, RICARDO; MARCHINI, TIMOTEO; D'ANNUNZIO, VERONICA; EVELSON, PABLO
Lugar:
Ciudad de Buenos Aires
Reunión:
Congreso; LXII Reunión Científica Anual; 2018
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
The exposure to environmental particulate matter (PM) is associated with increased morbidity and mortality rates due to several cardiovascular diseases, including stroke and myocardial infarction. Thioredoxin (Trx) is a key antioxidant system that plays an important role against oxidative stress; it regulates redox homeostasis and has cardioprotective effects reducing infarct size. A byproduct of fossil fuel combustion known as Residual Oil Fly Ashes (ROFA) is frequently used as a PM surrogate to evaluate the biological effects of the exposure to air pollution. Therefore, the aim of this work was to evaluate the role of Trx after an acute exposure to ROFA and clarify the mechanisms by which PM inhalation triggers cardiovascular adverse health effects. Wild type mice (WT) and transgenic mice overexpressing Trx-1 in heart (TRX) were intranasally instilled with a ROFA suspension (1 mg/kg) or saline solution (control group), and hearts were removed after 3 hours. Heart O2 consumption was significantly increased by 39% in TRXROFA mice compared to WTROFA (WTROFA: 1040 ± 90 ng-at O/min.g tissue, p<0.05). No differences were found in GSH levels, but an increase was observed in GSSG levels in TRXROFA group (WTCONTROL: 0.87 ± 0.10 µg GSSG/mg tissue, p<0.05), which lead to a decrease in the GSH/GSSG ratio in TRXROFA. Moreover, SOD activity was decreased in TRXROFA mice compared to WTROFA and TRXcontrol animals (WTROFA: 3.4 ± 0.6 USOD/mg protein, p<0.05). Oxidative damage to lipids was evaluated by the TBARS assay. A decrease was observed in TRXROFA mice when compared with WTROFA mice (460 ± 10 pmol/mg protein, p<0.01). The present findings indicate that overexpression of Trx-1 confers cardioprotection after an acute exposure to environmental PM, and therefore contribute to the understanding of the adverse health effects triggered by PM inhalation.