IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MITOCHONDRIAL FUNCTION IN THE BRAIN CORTEX OF RATS IN AN EXPERIMENTAL GLAUCOMA MODEL
Autor/es:
REIDES, CG; EVELSON, PA; FERREIRA, SM; LASAGNI VITAR, RM; GARCÉS, MS; LLESUY, SF; HVOZDA ARANA, AG; CALABRÓ, MV; LERNER, SF
Lugar:
Mar del Plata
Reunión:
Congreso; LXIII Reunión de la Sociedad Argentina de Investigaciones Clínicas (SAIC).; 2018
Institución organizadora:
Sociedad Argentina de Investigaciones Clínicas (SAIC).
Resumen:
Glaucoma is a disease characterized by a specific pattern of opticnerve head damage, retinal ganglion cells death and traditionallyassociated with high intraocular pressure. The understanding of therole of mitochondria in the onset and progression of glaucomatousdamage is important to the comprehension of this pathology sincethe function of the mitochondria exerts an important impact in theneuronal life or death pathway and is essential for neurotransmission.The aims were to evaluate the alterations of mitochondrial functionand antioxidant defenses in an experimental glaucoma model.Three-month female Wistar rats were divided in two groups (n=4):glaucoma group in which rats were operated under a microscopeby cauterized two of the episcleral veins (GG) and control groupwhich received a sham procedure (CG). Seven days after surgeryrats were euthanized, brain cortex was separated, and mitochondriawere isolated. The local committee for animal care (CICUAL-FFyB)approved the experimental model. The following markers were evaluated: oxygen consumption (OC), ATP production, hydrogen peroxide production, protein carbonylation (PC), and the activities ofthe enzymes superoxide dismutase (SOD), glutathione peroxidase(GPx), glutathione reductase (GR), glutathione S-transferase (GST)and thioredoxin reductase (TrxR).In GG the ATP production decreased 38% compared to CG (C 52.0± 4.4 nmol ATP/ min mg prot p<0.05) and protein carbonylation increased 60% compared to CG (C 4.28 ± 0.74 nmol/mg prot p<0.05).No significant differences were found in OC, hydrogen peroxide production, SOD, GPx, GR, GST, and TrxR.The results suggest that glaucoma produces an alteration of braincortex mitochondrial function characterized by a decrease in its capacity to produce ATP. Since no changes were observed in OC, thissituation could be a consequence of mitochondrial membrane potential alterations. Additionally, protein oxidation could contribute toaltered mitochondrial function, leading to changes in protein functionor inactivation.