FUNCTION OF USP9X AS A NEW PLAYER OF AUTOPHAGOSOME BIOGENESIS IN VMP1-DEPENDENT AUTOPHAGY.

TAMARA ORQUERA; MARIANA TADIC; FELIPE RENNA; MARÍA INÉS VACCARO

Mar del Plata

Congreso; SAIC. SAI. SAFIS. 2018; 2018

SAIC, SAI y SAFIS

Intracellular activation of zymogen granules leads to acute pancreatitis by pancreas self-digestion. Most of acute pancreatitis cases are self-limited, suggesting the importance of protective mechanisms of acinar cells. VMP1 is an autophagy-related protein whose expression is induced by pancreatitis and essential for autophagosome biogenesis. VMP1 mediates zymophagy, a selective type of autophagy-mediated degradation of zymogen granules that prevents acute pancreatitis. Previously we demonstrated that the deubiquitinase USP9x interacts with VMP1 during zymophagy. The aim of this work is to elucidate the function of USP9x in autophagosome formation. By immunofluorescence, USP9x presents a cytoplasmic diffuse pattern at basal conditions, and it relocates after 15 min of starvation to a perinuclear area (48.0% ± 1.2 vs 40.5% ± 1.9 p