ENDOTOXEMIA INDUCES MITOCHONDRIAL DYSFUNCTION AND MODIFIES MITOCHONDRIAL DYNA

SETAU, EMANUEL; ALVAREZ, SILVIA; VICO, TAMARA; VACCARO, MARÍA INÉS; VANASCO, VIRGINIA; MARCHINI, TIMOTEO

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigaciones Clínicas; 2018

SAIC

Pancreas has been described to be affected in early-endotoxemic process. Since mitochondria are the main source of ATP it is essential to maintain a suitable mitochondrial bioenergetics to recover from cellular, tissue and pancreatic organic damage in this syndrome. The aim was to analyze the status of pancreatic mitochondrial function and dynamics in a rat model of low grade and severe endotoxemia. Female Sprague Dawley rats (45 days) were ip injected by 0.5 mg/kg (LPS 0,5), by 8 mg/kg (LPS 8), or vehicle (control), and after 6 h assays were performed in pancreas. To evaluate mitochondrial function, mitochondrial oxygen consumption, ATP production and mitochondrial complex activities were measured. ATP production was found significantly decreased (30%) in both LPS-treatments (control value: 70.4 ± 5 nmoles ATP/min mg protein, p