IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The blockade of low affinity neurotensin receptor (NST2) disassembles neuronal membrane association between nNOS, PSD-95 and NMDA receptor. Evidences of mitochondrial dysfunction
Autor/es:
S. LORES ARNAIZ; J. MIRANDA; A. GUTNISKY; A.G. KARADAYIAN; G. RODRÍGUEZ DE LORES ARNAIZ
Lugar:
La Plata. Pcia de Buenos Aires
Reunión:
Congreso; XLVII Reunión Anual Sociedad Argentina de Biofísica, La Plata, Buenos Aires, Argentina, 4-7 de diciembre de 2018.; 2018
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
Previous results from our laboratory have shown impairment in neuronal nitric oxide synthase (nNOS) activity and expression after the blockade of low affinity neurotensin receptor (NST2) by levocabastine administration. Evidences show that post-synaptic density protein 95 (PSD-95) links nNOS with the N-methyl-D-aspartic acid (NMDA) receptor in CNS. Male Wistar rats were i.p. injected at a single dose with levocabastine (50 μg/kg) or saline and were decapitated 18 hours later. Crude mitochondrial fractions from cerebral cortex were obtained by differential centrifugation, while synaptosomal membrane fractions were isolated by differential and sucrose gradient centrifugation. Results from Western blot assays indicated that the protein expression of PSD-95, NR2B subunit of NMDA and β-actin after levocabastine administration were respectively 72, 34 and 45% lower than those in vehicle injected samples (p