Sympathetic hyperactivity abolishes the myocardial protective effect of vagal stimulation in mice with cardiac GS alfa overexpression.

BERNATENÉ EA; GELPI RJ; MÉNDEZ DIODATI N; BUCHHOLZ B; KELLY J; CASANOVA VC

Mar del Plata.

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica.; 2018

Sociedad Argentina de Investigación Clínica.

(498) Although important myocardial protective effects of vagal stimulation (VS) have been demonstrated experimentally, the clinical results are not entirely conclusive. We hypothesized that the dysautonomia that accompanies heart disease may interfere with myocardial protection. The objective was to study whether sympathetic cardiac hyperactivity abolishes the protective effects of classical preconditioning and preconditioning mimicked by VS in transgenic mice with cardiac overexpression of GSα protein.Transgenic FVB mice with specific cardiac overexpression of the GSα protein (TG) and their respective non-transgenic controls (WT) were subjected to 30 min of regional ischemia and 2 hours of reperfusion (I/R). Both TG and WT mice received VS for 10 min before ischemia or 3 preconditioning cycles for 5 min of ischemia and 5 min of myocardial reperfusion (PC) before prolonged ischemia (n= 5-7 per group). All animals were catheterized to measure ventricular function. Hearts were dyed with Evans Blue and incubated in TTC to measure the infarct size (IS).The heart rate (HR) of the WT and TG mice was 438±11 and 664±14 (p