IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PRELIMINARY EFFECTS OF MELATONIN ADMINISTRATION ON FE-DEPENDENT RESPONSE TO OXIDATIVE STRESS IN RAT BRAIN
Autor/es:
COGO PAGELLA, J.; HERNANDO, M. P.; PUNTARULO, S.; PILONI, N.E.; CERVINO, C. O.
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión conjunta de sociedades: SAIC, SAI, SAFIS; 2018
Institución organizadora:
SAIC
Resumen:
Melatonin (ML) is considered as a quencher of free radicals generation.Its secretion is related to the photoperiod. The effect of MLon rats? brain was studied after acute Fe-dextran exposure. Ratswere ip administrated with Fe-dextran (500mg/kg) or saline solution(control), and after 6 h ML (0, 50 and 100 mg/kg) was ip injectedand the brain was removed 2 h later. Endogenous ML productionwas changed by exposing the rats to normal photoperiod, 12:12h(control), 15 days of 24h light (L), or 24 h darkness (D). At the endof each photoperiod, the Fe-dextranor saline solution was administrated.After 8 h, thiobarbituric acid reactive substances content(TBARS), catalase activity (CAT) and glutathione content (GSH)were evaluated. In presence of Fe, no changes on TBARS and CATwere observed at any doses of ML administrated. GSH decreasedby 60 and 40% after the injection with 50 and 100 mg/kg ML, respectively,as compared to control. In the absence of Fe, when theincrease in ML content was due to exposure to D, no alterationswere observed in TBARS, an increase in GSH and a decrease inCAT activity was determined. Furthermore, in rats supplementedwith Fe, TBARS increased, GSH was not affected, and CAT activitydecreased, as compared to control photoperiod. However, GSH wassignificantly lower compared with absence of Fe. In the presenceof L, no changes were detected. These results suggested that MLeffects on oxidative status showed a different profile depending ofFe overload. Keywords: Melatonin, Fe acute exposure, TBARS, catalase,reduced glutathione.