Redox-sensitive transcription factors and glutathione metabolism in rat brain after acute fe-dextran treatment

CERVINO, C. O.; PILONI, N.E.; PUNTARULO, S.; HERNANDO, M. P.

Mar del Plata

Congreso; Reunión conjunta de sociedades: SAIC, SAI, SAFIS; 2018

SAIC

It was previously described that acute Fe-dextran treatment modifiedredox-sensitive transcription factor related to cell protectionagainst oxidative stress in rat brain. NF-κB DNA binding capacityand nuclear Nrf2 expression levels were affected after 6 h of Feadministration. However, no significant differences in the expressionof Keap 1 protein in nuclear or cytosolic fractions of brain extractswere observed. Cellular defense systems involve the expression ofantioxidant and detoxification genes, including glutathione S-transferase(GST) and hemeoxygenase 1, among others. The aim of thisstudy was to determine the activation of brain Nrf2/Keap1 signalingpathway, after acute Fe-dextran treatment to rats, assessing theparticipation of target genes. A single dose of 500 mg Fe-dextran/kg body weight was administered intraperitoneally to male SpragueDawley rats. Total brain samples or cortex area were obtained fromcontrol and treated animals at 6 and 8 h post injection (pi). GSTactivity and total thiols were determined spectrophotometrically. Glutathione(GSH) content was determined by reverse phase HPLC.Results indicate that GST activity was 2-fold increased at 8 h pi withrespect to control values (p