IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of pre-ischemic vagal stimulation on the infarct size and left ventricular remodeling in a myocardial ischemia and reperfusion mice model
Autor/es:
BUCHHOLZ B; LAGO N; ARTANA CL; DONATO M; FRANCO RIVEROS V; ELIANA CICALE; GELPI RJ
Reunión:
Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018
Resumen:
The objective was to study the effects of brief vagal stimulation (VS)applied before ischemia on acute myocardial infarction and its longtermevolution, analysing the left ventricular remodeling in an experimentalischemia/reperfusion model. Mice were randomly assignedto different protocol groups (n=5-6). Animals underwent regionalmyocardial ischemia during 45 min, followed by either 2 h or 28 daysof reperfusion, with or without 10 min of pre-ischemic vagal stimulation(VS) and every protocol had their respective Sham group. Thefollowing parameters were measured: left ventricular function (LVF),by catheterization of the carotid artery; infarct size (IS) by TTC; EjectionFraction (EF), Shortening Fraction (SF), Isovolumetric RelaxationTime (IVRT) by echocardiography and morphometric analysiswas determined by comparing left ventricle weight with the length ofthe tibia (LVW/LTi) and the tail (LVW/LT). VS+IR-2h had smaller IScompared to IR-2h (45±2% and 67±3%, respectively, p<0.001). IR-28d group showed a LVEDP of 6.91±1 mmHg which is significantlyhigher than Sham-28d (3.81±0.2 mmHg; p<0.01). Likewise, thereis a decrease in the EF (IR-28d: 58.8±3.1 vs Sham-28d: 74±1.6,p<0.01), SF (IR-28d: 32.7±2.6 vs Sham-28d: 35.5±1.4, p<0.01), anda difference in the IVRT (IR-28d: 30.3±1.2 vs Sham-28d: 21.3±1.1,p<0.001). Pre-ischemic VS improves this data: EF (VS+IR-28d:68.6±3.7 vs IR-28d, p<0.05), SF (VS+IR-28d: 32.7±2.6 vs IR-28d.p=n.s.) and IVRT (VS+IR-28d: 23.7±1.2 vs IR-28d. p<0.01). Finally,data on LVW/LTi in Sham-28d was 4.81±0.06 while in IR-28d raisedto 5.64±0.4 (p<0.05) and didn?t changed in VS+IR-28d (5.71±0.3,p=n.s.) In conclusion, VS applied before ischemia confers cardioprotection,reducing the acute infarct size and improving long-termLVF.