NADPH oxidase contributes to redox imbalance in raw 264.7 murine macrophages exposed to air pollution particulate matter

CONTIN, MARIO; MAGNANI, NATALIA; GONZALEZ MAGLIO, DANIEL; CACERES, LOURDES; GARCÉS, MARIANA; TASAT, DEBORAH; MARCHINI, TIMOTEO; PAZ, MARIELA; CALABRO, VALERIA; TRIPODI, VALERIA; EVELSON, PABLO

Mar del Plata

Congreso; LXII Reunión Científica Anual; 2018

Sociedad Argentina de Investigación Clínica

The exposure to environmental particulate matter (PM) induces pulmonary oxidative stress and inflammation. Alveolar macrophages are suggested to play a central role in this scenario, since they produce inflammatory mediators and reactive oxygen species (ROS) following PM uptake. The aim of our work was to characterize this inflammatory response and to address the main targets of the redox imbalance observed in macrophages after the exposure to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. The murine cell line RAW 264.7 was exposed to ROFA at 25, 50, or 100 μg/mL for 24 h. Cell viability was not significantly affected under these experimental conditions. Cell culture supernatants showed increased TNF-α levels after incubation with ROFA 100 μg/mL (control: 480±140 pg/mL TNF-α, p