Assessment of the antioxidant activity of Aloysia citrodora and Lippia turbinata extracts

REIDES C; FERREIRA S; RICCO R; CARBALLO M; LLESUY S

Chile

Congreso; Free radicals and antioxidants; 2009

Society for free radical biology and medicine

  The aim of this work was to evaluate the antioxidant capacity, the effect on lipid peroxidation and on protein oxidation of Aloysia citrodora (AC) and Lippia turbinata  (LT) extracts . Decotion (D) and infusion (I) of both AC and LT were used at final concentration of 5 % w/v. Brain homogenates and plasma were used to evaluate the effect on lipid peroxidation and to determine the protection over protein carbonylation (PC) respectively.  Peroxidation was quantified by means of spontaneus chemiluminescence (CL) and thiobarbituric acid test (TBARS). For the determination of the in vitro total antioxidant capacity, the following assays were used: TRAP, ABTS and   DPPH. PC decreased   72 (DAC), 29 (IAC), 96 (DLT) and 94 (ILT), (control value (CV): 0.78 ± 0.08 nmol/mg protein). 50 % i nhibition (IC50)   of CL was observed with: 0,09 mL  DAC, 0,14 mL IAC, 0,32 mL DLT and 0.48mL  ILT (CV: 723 ±  41 cpm/ mg protein). TBARS were decreased 74 DAC, 54 % IAC, 65 (DLT) and 21 % (ILT), (100% oxidized homogenates: 0,92  ± 0,10 nmoles/ mg protein). Values for total antioxidant capacity were: a-TRAP: 24, 18, 3,8  and 3,7 mM for DAC,  IAC, DLT and ILT; b-DPPH: 12, 12, 3,2 and 2,9 mM for DAC,  IAC, DLT and ILT c- ABTS: 26, 18, 4,7 and 3,4  mM for DAC,  IAC, DLT and ILT. The results obtained in the present study clearly demonstrate that both extracts have antioxidant properties, being D more efficient than I. Furthermore, AC has proven to be four times more effective than LT, suggesting their interest as ingredients for antioxidants formulations.