DIFFERENT MECHANISMS OF CARDIOPROTECTION IN REMOTE ISCHEMIC PRE AND POSTCONDITIONING

PAEZ D; GELPI RJ; FRANCO RIVEROS V; BUCHHOLZ B; BIN EP; GRECO C; DONATO M

Mar del Plata

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2018

Sociedad Argentina de Investigación Clínica

Background: It is known that brief episode(s) of ischemia/reperfusionapplied prior or after myocardial ischemia to peripheral tissuelocated at a distance from the heart significantly reduce myocardialinfarct size. This phenomenon is called remote ischemic conditioning(RIc).Objective: The aim was compared the efficacy of RIc in protectingthe heart when the RIc stimulus is applied prior to myocardial ischemia(remote ischemic preconditioning; rIPC), or at the onset ofmyocardial reperfusion (remote ischemic postconditioning; rIPost).Methods: In order to induce myocardial infarction, we used mice(FVB, 24 g) which were subjected to 30 min of left coronary arteryocclusion followed by 120 min of reperfusion. rIPC and rIPost wereinduced by 3 cycles of 5 min of ischemia followed by 5 min of reperfusion of the left femoral artery. The infarct size was measured usingEvans blue (Risk Area) and Triphenyl-tetrazolium chloride (Infarctsize).Results: The ischemia/reperfusion protocol (30 min of ischemia and120 min of reperfusion) induced an infarct size of 69.13±3.22 %.The rIPC and rIPost significantly reduced infarct size to 51.17±4.36(p