Thiol redox homeostasis in visual cortex in an experimental glaucoma model

PEVERINI A; LASAGNI VITAR RM; LLESUY SF; REIDES CG; JANEZIC N; FERREIRA SM; HVOZDA ARANA A; LERNER F,

Ciudad Autónoma de Buenos Aires

Congreso; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2017

Alterations in thiol redox homeostasis have been implicated in the pathology of neurodegenerative diseases. Recent evidence has detected neurodegenerative lesions in glaucomatous optic nerve, lateral geniculate nucleus and visual cortex, suggesting that glaucoma should be considered a neurodegenerative disorder.The aim of this work was to evaluate thiol redox homeostasis in visual cortex in a glaucoma rat model. Female Wistar rats (3 months) were divided in two groups (n=12): glaucoma in which rats were operated under a microscope by cauterized two of the episcleral veins (G) and control which received a sham procedure (C). Seven days after surgery rats were euthanized, brains were removed and visual cortex was separated.The following markers were evaluated: glutathione reductase (GR), glutathione peroxidase (GPx), reduced glutathione (GSH), oxidized glutathione (GSSG), redox index (GSH/GSSG), glucose-6-phosphate deshidrogenase (G6PD) and thioredoxin reductase (TrxR)Comparing glaucoma to control: GR diminished 52% (C: 13.0 ± 1.0 nmol/min.mg protein p