VMP1 mediated zymophagy is triggered by caerulein treatment in differentiated AR42J cells

DANIEL GRASSO; MARÍA INÉS VACCARO; FELIPE RENNA; TAMARA ORQUERA

CABA

Congreso; PANCREAS 2017; 2017

International Association of Pancreatology / European Pancreatic Club

Introduction: Autophagy is a cellular degradative process capable to sequester and degrade even entire organelles. VMP1 is an autophagy proteins that mediates the selective degradation of activated zymogen granules during acute pancreatitis (zymophagy) in humans and animal models .Aim: To describe the zymophagic process in a cellular model of acute pancreatitis based in dexamethasone-differentiated AR42J (AR42J-Dex) cell line.Results: Experiments were made in AR42J-Dex treated with Caerulein (Cae), 1uM, at 0, 10, 15, 20, 25, 30 minutes. An increase of VMP1 was observed during the course of treatment. Autophagy, evaluated by LC3, was detected in response to the Cae treatment. Moreover, a clear LC3 redistribution was observed with Cae treatment. Colocalization among VMP1, LC3 and trypsin could be observed in response to the pancreatitis model. Finally, in response to Cae, zymogen granules, marked by amylase immunofluorescence, were observed in colocalization with ubiquitin and VMP1.Conclusion: Results suggest that zymophagy is activated even in a cellular model of acute pancreatitis. These results present an useful tool for the study of autophagy during acute pancreatitis towards a better therapeutic of this disease.