THE ROLE OF MITOCHONDRIA IN THE OXIDATIVE RESPONSE OF RAW 264.7 MURINE MACROPHAGES EXPOSED TO AIR POLLUTION PARTICULATE MATTER

LOURDES CACERES; MARIANA GARCES ; VALERIA CALABRO ; SILVIA ALVAREZ ; MARIELA PAZ ; PAMELA MARTINO ADAMI ; DIEGO OJEDA ; DEBORAH TASAT; DANIEL GONZALEZ MAGLIO ; TIMOTEO MARCHINI ; LAURA MORELLI ; PABLO EVELSON

Buenos Aires

Congreso; LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2017

Reunion Conjunta de Sociedades de Biociencias

The exposure to environmental particulate matter (PM) is associatedwith pulmonary oxidative stress and inflammation. Alveolarmacrophages are suggested to play a central role in this scenario,since they produce inflammatory mediators and reactive oxygenspecies. The aim of this work was to study the redox status and therole of mitochondria in macrophages after the exposure to ResidualOil Fly Ash (ROFA), a PM surrogate rich in transition metals. Themurine cell line RAW 264.7 was exposed to ROFA at 100 μg/mL for24 hs. Cell viability was not significantly affected under these experimentalconditions. Intracellular redox status was assessed by flowcytometry as dichlorofluorescein mean fluorescence intensity (MFI),which was increased by 30% following ROFA incubation comparedto the control group (p