Preliminar study of redox-sensitive transcription factors in rat brain after acute Fe-dextran treatment.

PUNTARULO S.; PILONI NE; FERNANDEZ V.; VARGAS R; VIDELA L.A

Buenos Aires

Congreso; Reunión conjunta de sociedades de Biociencias: SAIC, SAIB, SAI, SAFE, SAH, SAP, SAB, SAFIS, SAA; 2017

SAIC

The aim of this study was to determine the effect of acute Fe administration on the rat brain Nrf2/Keap1 signaling pathway. It was reported that acute Fe-dextran treatment increased rat brain Fe content after 6 h of administration, and modified the NF-κB DNA binding capacity triggering an enhancement in the activity of antioxidant enzymes (such as catalase). Nrf2, constitutively repressed due to its binding to the cytoplasmic Kelch-like ECH associated protein 1 (Keap1) protein, is a redox-sensitive transcription factor related to cell protection against oxidative stress. Nrf2 function is primary controlled by its subcellular distribution and the effect of cellular Fe content is not clearly established yet. The activity of catalase seems as a target for the nuclear factor erythroid 2-related factor 2 (Nrf2) gene products. Fe-dextran treatment was performed as the intraperitoneally administration of a single dose of 500 mg Fe/kg body weight to male Sprague Dawley rats. Brain samples were obtained both from control and treated animals after 4, 6 and 8 h of Fe-dextran injection. Cell expression of both factors was determined by Western Blot. It was observed a significant increase in nuclear Nrf2 expression levels after 6 h of Fe administration in rat brain with respect to control values (p