Overexpression of thioredoxin in heart has a protective effect after exposure to ambient air particles

GARCÉS M; TASAT D; ALVAREZ S; MARCHINI T; D´ANNUNZIO V; GUAGLIANONE A; EVELSON P; CALABRÓ V; GELPI RJ

Buenos Aires

Congreso; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2017

Reunion Conjunta de Sociedades de Biociencias

The exposure to environmental particulate matter (PM) is associatedwith increased morbidity and mortality rates due to severalcardiovascular diseases, including stroke and myocardial infarction.Thioredoxin (Trx) is a key antioxidant system that plays an importantrole against oxidative stress; it regulates redox homeostasis and hascardioprotective effects reducing infarct size. A byproduct of fossilfuel combustion known as Residual Oil Fly Ashes (ROFA) is frequentlyused as a PM surrogate to evaluate the biological effectsof the exposure to air pollution. Therefore, the aim of this work wasto evaluate the role of Trx after an acute exposure to ROFA andclarify the mechanisms by which PM inhalation triggers cardiovascularadverse health effects. Wild type mice (WT) and transgenicmice overexpressing Trx-1 in heart (TRX) were intranasally instilledwith a ROFA suspension (1 mg/kg) or saline solution (control group),and hearts were removed after 3 hours. Heart O2 consumption wassignificantly increased by 39% in TRXROFA mice compared to WTROFA(WTROFA: 1040 ± 90 ng-at O/min.g tissue, p