IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Nitric oxide (NO) in white adipose tissue of high fat fed mice: effect of dietary (-)-epicatechin
Autor/es:
TOBLLI, JE; HID, E; PIOTRKWOWSKI, B; PRINCE, PD; GALLEANO, M; FISCHERMAN, L; LITTERIO, MC; FRAGA, CG
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión conjunta de sociedades de biociencias; 2017
Institución organizadora:
SAIC-SAIB-SAI-SAA-SAB-SAB-SAFE-SAFIS-SAH-SAP
Resumen:
Changes in NO metabolism in white adipose tissue cells are commonduring the development of obesity and metabolic syndrome.(?)-Epicatechin (EC) is a flavonoid present in fruits and vegetableswith the potential to avoid or attenuate the alterations in NO productionand/or degradation.The aim of this work was to analyze adipose tissue response to ahigh-fat diet supplemented with EC, in terms of fat pads remodelingand NO metabolism. For 15 w, two experimental groups of malemice (C57BL/6) received control diet, with (CE) or without EC supplementation(C). Two other groups received HFD (60% of caloriesfrom fat), with EC (HFE) or without EC supplementation (HF). Food,energy intake and body weight was evaluated during the experimentalperiod. At the end, the increase in body weight (BW) inducedby high-fat diet was similar, independently of EC supplementation(4.0±0.3 g). The four fat pads isolated showed significant increasesin their weight relative to BW in HF respect to C (47% in perirenal,72% retroperitoneal, 84% mesenteric and 55% epidydimal, p˂0.05).EC supplementation slightly attenuated this effect in retroperitonealand mesenteric pads. Expression of endothelial, neuronal andinducible NO synthases (eNOS, nNOS and iNOS) and activatorphosphorylation of eNOS in serine 1177 were determined in thefat pads, as well as the levels of nitrotyrosine-containing proteins,a hallmark of peroxynitrite production. NOS expressions were notaffected by the treatments. In retroperitoneal pad, EC was able toactivate eNOS via its phosphorylation regardless of the presenceof high fat content in the diet. Additionally, nitrotyrosine-containingprotein levels were significantly lower in HFE group respect to HF(-20%, p