Metalloproteinase activity in heart is increased in mice treated with a low dose of corticosterone

DANNUNZIO V; SCHREIER L; MAZO T; FABRE B; FERNANDEZ MACHULSKY NH; GELPI RJ; BERG G

buenos aires

Congreso; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2017

Sociedades de biociencias

Continuous increase of glucocorticoids due to chronic activation ofthe pituitary axis is one of the consequences of chronic stress. Theknowledge of its effects on plaque vulnerability markers and cardiovasculardisease will help to elucidate different pathophysiologicalmechanisms. With this aim, we studied 15 C57BL/6j mice treated(TM) for 4 weeks with 35 μg/ml corticosterone and 15 control mice(CM). After euthanasia, heart, liver, adrenal glands, epididymal andintestinal adipose tissue (AT) were weighed. Serum lipid profile wasevaluated. We performed a zymography of homogenized cardiactissue and epididymal AT for measuring metalloprotease activity(MMPs). In isolated and isovolumetric hearts perfunded with Langendorfftechnique, we measured the left ventricle develop pressure(LVDP) and its response to ß adrenergic stimulus (Isoproterenol (iso)1 μM). Results: At the end of treatment, adrenal gland weight waslower in TM (0.002 (0.0002) vs 0.003 (0.0004) gr, p=0.03). Weightgain was observed in TM vs CM (5.0 (1.5-9.5) vs 3.3 (2.0-7.0) gr,p=0.007), at expense of epididymal AT (0.73 (0.06) vs. 0.33 (0.04) g,p