Chronic exposure to particulate ambient air pollution from Buenos Aires City alters oxidative metabolism in mouse brain cortex.

MARCHINI, TIMOTEO; CACERES, LOURDES; EVELSON, PABLO; CALABRO, VALERIA; GUAGLIANONE, ALEJANDRO; GARCÉS, MARIANA; ALVAREZ, SILVIA; BERRA, ALEJANDRO

Ciudad de Buenos Aires

Congreso; LXII Reunión Científica Anual; 2017

Sociedad Argentina de Investigación Clínica

Increasing evidence indicates that the central nervous system is a target of air pollution, causing alterations such as neuroinflammation, and neurodegeneration. However, the mechanisms mediating these effects have not yet been fully elucidated. The aim of this work was to study the effects of chronic exposure to ambient air pollution on mice brain cortex oxidative parameters. C57BL/6J mice were continuously exposed to polluted air from a central area of Buenos Aires city for 7, 15 or 30 days. Mice were housed in chambers specially designed for that purpose. Control mice were subjected to the same protocol but interposing filters to the air flux to the chamber in order to retain particulate matter. TBARS, carbonyl and glutathione levels were measured in brain cortex homogenates as oxidative stress markers. SOD and NOX activities were also assesed in order to evaluate superoxide anion metabolism. Lipid oxidative damage, measured as TBARS, was increased after 7 days of polluted air exposure compared to control mice (36%, p