CONICET | Buscador de Institutos y Recursos Humanos

Pancreatic lipase (PL) is the key enzyme involved in triglyceride absorption in the small intestine. Inhibiting PLactivity is a possible approach to prevent dyslipidemias and/or obesity.Peanut skin is a waste during the manufacturing of some peanut products and can be the source of potential bioactivecompounds. However, the industrial method used to process the peanuts can affect the composition of the skins andthe biological activities. The aim of this work was to determine the PL inhibitory activity of extracts and purifiedfractions of peanut skins subjected to blanching or roasting and to associate it with their chemical composition.Peanuts (type Runner from Argentina, crop 2012) were subjected to blanching (90ºC, 10 min) or roasting (150ºC, 20min). Peanuts skins were extracted by using ethanol-water (70:30 v/v) obtaining the crude extracts BCE and RCE(from blanching and roasting processes, respectively). From them were obtained water fractions (BW and RW) andethyl acetate fractions (BEA) and (REA). Inhibition of PL was tested by monitoring the cleavage of 1,2-o-dilaurylrac-glycero-3-glutaricacid (6-methyresorufin) ester to produce methylresorufin in the absence and the presence of 0?2500 μg/ml of the different extracts/fractions. Orlistat was used as a positive control. BCE was 10-20% more potentthan RCE as PL inhibitor at all the tested concentrations. Water fractions (BW and RW) were weaker PL inhibitorsthan ethyl acetate fractions (BEA and REA). BEA showed a significant lower IC50 respect to REA: 21 ug/ml and 72ug/ml for BEA and REA, respectively (p B > P, and correlatedwith the presence of procyanidin dimer type A, and in a less extent with procyanidin dimer type B.These results suggest that peanut skin obtained after blanching can be a useful food ingredient with the potentiality ofdecrease triglyceride absorption in humans.