OXIDATIVE AND INFLAMMATORY RESPONSE OF RAW 264.7 MURINE MACROPHAGES EXPOSED TO AIR POLLUTION PARTICULATE MATTER

GARCES M; GONZALEZ MAGLIO DH; PAZ ML; TASAT DR; EVELSON PA.; CACERES L; OJEDA D; MARCHINI T

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2016

Theexposure to environmental particulate matter (PM) is associated withincreased cardiorespiratory morbidity and mortality rates. Alveolar macrophagesare suggested to play a central role in this scenario, triggering bothinflammation and oxidative stress following PM inhalation. However, the pathways linkingthese responses remain uncertain. In order to address such mechanisms, westudied the murine macrophage cell line RAW 264.7 after an exposure todifferent PM samples, Residual Oil Fly Ash (ROFA) andConcentrated Ambient Particles (CAPs), at 0, 50, 100, and 200 µg/mL for 24 h. Intracellular redox status was assessed bydichlorofluorescein fluorescence, which was increased in a dose-dependentmanner by up to 73% and 71% at 200 µg/mL of ROFA and CAPs, respectively(control: 16±5 x 103) AU, P