IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Oxygen metabolism in the cardiorespiratory system after an acute exposure to Ni-doped nanoparticles
Autor/es:
MAGNANI, NATALIA; GUAGLIANONE, ALEJANDRO; DIAZ, LUIS; GARCÉS, MARIANA; CACERES, LOURDES; DESIMONE, MARTIN; EVELSON, PABLO; MARCHINI, TIMOTEO; MEBERT, ANDREA; ALVAREZ, SILVIA
Lugar:
Montevideo
Reunión:
Simposio; Simposio: Protein oxidation and turnover: relevance in biology and medicine?; 2016
Institución organizadora:
Facultad de Medicina, Universidad de la República.
Resumen:
There is strong evidence that ambient air pollution particles present a serious risk to human health. Numerous epidemiological studies have shown an association between airborne particulate matter (PM) and increased cardiopulmonary morbidity and mortality. Transition metals are frequent PM constituents and might play a central role in cardiopulmonary disease mechanisms initiated by PM exposure, via increased production of reactive oxygen species by Fenton-like chemical reactions. The aim of this work was to analyze lung and heart oxidative metabolism after an acute exposure to Ni-doped silica nanoparticles (Ni-NP), in order to clarify the molecular mechanisms involved in PM toxicity. Swiss mice (20-25 g) were intranasally instilled with a Ni-NP suspension (1.0 mg Ni/kg body weight), delivered in a single dose. The control group was exposed to a silica NP without Ni. Lung, heart, and blood samples were collected 1 h after the exposure. NP share comparable physicochemical properties with air pollution PM in size (Control: 170 ± 2 nm; Ni-NP: 200 ± 20 nm) and shape, as assayed by TEM. The Ni-NP group showed an increase in tissue O2 consumption (67%; p˂0.001) and NADPH oxidase (Nox) activity (41%; p˂0.001) when compared with the control group. Moreover, GSH/GSSG ratio was decreased in the treated group (p˂0.05). Ni-NP mice also showed an increase in TBARS content in lung (35%; p˂0.001). Plasma TBARS level was increased by 54% (p˂0.001) in Ni-NP group. In heart samples, O2 consumption rates were decreased in Ni-NP mice (37%; p˂0.0001). Taken together, the present data indicate that the exposure to Ni-NP induces impaired oxidative metabolism in lung and heart. These findings contribute to the understanding of the cardiopulmonary toxicity of PM exposure, where oxidative stress and inflammation may play a predominant role in association with transition metals present in environmental PM.