Effects of diesel exhaust particles (DEP) on the redox balance of human conjunctival epithelial cells.

TAU, J; TESONE, AI; BERRA, A; LASAGNI VITAR, RM; HVOZDA ARANA, AG; REIDES, CG; LLESUY, SF; JANEZIC, NS; PEVERINI, A; FERREIRA, SM

Mar del Plata

Congreso; LXI Reunión de la Sociedad Argentina de Investigaciones Clínicas (SAIC).; 2016

Sociedad Argentina de Investigaciones Clínicas (SAIC).

Airpollution causes adverse effects on human health and the eyes are particularlyvulnerable to these effects, as they are constantly exposed to the environment.The aim of the present study was to evaluate the redox balance in humanconjunctival epithelial cells (IOBA-NHC) after the incubation with dieselexhaust particles (DEP) for 24 hours.The incubation was performed at differentconcentration of DEP (10, 50, and 100 μg/mL) for 24 hours and the following parameterswere evaluated: the activities of antioxidant enzymes glutathione reductase(GR), glutathione S-transferase (GST), glutathione peroxidase (GPX),thioredoxin reductase (TRxR) and glucose 6P-deshidrogenase (G6PDH); and reducedglutathione (GSH) and oxidized (GSSG) levels. One-way ANOVA test and Dunnett?stest as post hoc test were used for statistical analysis.The cells exposed to 50 and 100 μg/mL of DEP showed a significant decreasein GR (17 % and 37 %, p