IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CARDIAC BIOENERGETICS AND MITOCHONDRIAL PATHWAYS IN A RAT MODEL OF LOW AND SEVERE ENDOTOXEMIA.
Autor/es:
VICO TAMARA; JUAN SANTIAGO ADÁN AREÁN; MARCHINI TIMOTEO; ALVAREZ SILVIA; VANASCO VIRGINIA; EVELSON PABLO
Lugar:
Mar del Plata
Reunión:
Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016
Institución organizadora:
Sociedad Argentina de Investigaciones Clínicas
Resumen:
Despite being the major source of energy in the cell, mitochondriaparticipate in multiple cell signaling pathways. Besides,mitochondria dysfunction is suggested to play a central role ininflammatory pathologies like metabolic, cardiovascular and neurologicaldiseases, and also in acute endotoxemia and sepsis.The aim of this study was to analize cardiac bioenergetics andmitochondrial function in low and severe endotoxemia. FemaleSprague-Dawley rats (45 days old) were separated in three experimentalgroups and i.p. injected with a single dose of LPS (0.5or 8 mg/kg) or vehicle. After 6 h of treatment, mitochondria wereisolated from left ventricle. O2 uptake in state 4 (rest respirationstate) and state 3 (active state) was measured and a 15% and24% decrease in state 3 was observed in LPS 0.5 and LPS 8 mg/kg groups, respectively (Control: 74.8±22 ng-atO/min.mg protein).Complex I-III activity showed a 15% decrease at the highest doseof LPS (Control: 92.1±14 nmol/min.mg protein). Complex II-III andIV activities showed no statistical differences. ATP productionwas observed decreased by 33% at the highest dose (Control:186.2±55 nmol ATP/min.mg protein, p