IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Alterations in mitochondrial bioenergetics and nitric oxide metabolism due to acute alcohol exposure in mouse brain cortex synaptosomes and non-synaptic mitochondria
Autor/es:
ANALIA CZERNICZYNIEC; LORES-ARNAIZ SILVIA; ANALIA G. KARADAYIAN; JUANITA BUSTAMANTE; PAULINA LOMBARDI
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2016
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
The abuse of alcohol consumption is a type of alcohol-related disorder causing a number of serious consequences to health. Recently, we demonstrated that alcohol hangover (AH) induced a strong oxidative stress in brain cortex. Specifically, synaptosomes (S) resulted to be more affected due to AH than non-synaptic mitochondria (NSm). The aim of the present work was to study mitochondrial bioenergetics and NO metabolism at the beginning of AH in brain cortex NSm and S. Swiss male mice were treated with a single i.p. injection of saline (control group) or ethanol (3.8 g/kg; AH group). Biochemical assays were performed 6 h post-treatment when blood alcohol concentration was close to zero (AH start) and included mitochondrial respiration, respiratory complexes activity, mitochondrial transmembrane potential, UCP-2 and nNOS expression, NOS activity and NO total content. Results showed a significant decrease in respiratory function both subcellular fractions (p