IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
(-)-Epicatechin attenuates inflammation via NADPH oxidase and NF-κB modulation in kidney from high fructose-fed rats
Autor/es:
HID, E; GALLEANO, M; PRINCE, PD; OTEIZA, PI; FRAGA, CG; TOBLLI, JE
Lugar:
Norwich
Reunión:
Congreso; The 1st International Conference on Food Bioactives and Health; 2016
Institución organizadora:
FBHC 2016
Resumen:
High fructose consumption has been associated to deleterious metabolic conditions. In the kidney, fructose causes alterations that can contribute to chronic kidney disease; e.g. inflammation- associated renal damage and loss of renal function. We evaluated the capacity of (‒)-epicatechin to attenuate high fructose-triggered inflammation and NF-κB activation rat kidney cortex. Male Sprague-Dawley rats were fed for 8 w with standard diet (C); standard diet and 10% (w/v) fructose in the drinking water (F); and standard diet containing (‒)-epicatechin (20 mg/kg BW) and fructose-supplemented water (FE). (‒)-Epicatechin supplementation attenuated the fructose-induced increased expression of the inflammatory proteins iNOS, TNFα, and IL-6 in kidney cortex. The activation of several steps in the NF-κB pathway washigher in F than in C and FE: i) higher IKKα/β and p65phosphorylation, ii) higher IκBα phosphorylation, and iii) higher p65 nuclear/cytosolic ratio. However, no differences were observed in the expression of TLR4 among the groups. As oxidantscanalternatively activate NF-κB, we next measured superoxide production, and NADPH-oxidase (NOX) subunits expression in renal cortex. The F group showed a higher superoxide production associated with higher expressionsofp47phox and gp91phox NOX2 subunits, and NOX4, which were not observed in FE rats. Taken together, results suggest that in high fructose-fed rats the capacity of (‒)-epicatechinto inhibitinflammation and NF-κB activation is associated with the regulation of NADPH-oxidase increased expression and activity.