) CHANGES IN THE BRAIN REDOX STATUS IN A GLUTAMATE EXCITOTOXICITY MODEL

AILEN GALA HVOZDA ARANA ; CLAUDIA GABRIELA REIDES; SUSANA FRANSISCA LLESUY ; AGUSTINA PEVERINI; NATHALIE WEICHSLER ; NATASHA STEPHANIE JANEZIC; ROMINA MAYRA LASAGNI VITAR ; SANDRA MARIA FERREIRACA

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

Glutamate is an excitatory neurotransmitter in central nervous system. Glutamate excitotoxicity is thought to play an important role in neuronal damage. The aim was to evaluate changes in redox homeostasis in brain of rats subject to an experimental modelof glutamate excitotoxicity.The experimental model consisted of one group (n=6) injected ip with 1g glutamate/kg weight (GG) at days 1, 5 and 9 and control group (CG) injected with saline solution (n=6) at same days. The following markers were evaluated in brain homogenates: the activities of superoxide dismutase (SOD), catalase (CAT) thioredoxin reductase (TR), glutathione peroxidase (GPx), glutathione transferase (GT) glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6P), protein oxidation (PO), lipid damage (TBARS), ascorbic acid concentration (AA), and glutathione (GSH) levels. SOD activity was 5.90±0.10 U/mg protein in GG (CG 4.63±0.17 U/mg protein p