Time course of oxidative stress markers and inflammatory response of human conjunctival epithelial cells exposed to diesel exhaust particles (DEP).

TAU, J; HVOZDA ARANA, AG; FERREIRA, SM; LASAGNI VITAR, RM;; JANEZIC, NS; BERRA, A; TESONE, AI ; REIDES, CG; LLESUY, SF.

Congreso; . Annual Meeting of the Association for Research in Vision and Ophthalmology; 2016

The public health impact of airborne particulate matter shows adverse health effects at exposures that are currently experienced by urban populations. The aim of the present study was to evaluate oxidative stress markers and inflammatory mediators in human conjunctival epithelial cells after the incubation with diesel exhaust particles (DEP) for 1, 3 and 24 hours.Methods: The incubation was performed at different concentration of DEP (10, 50 and 100 μg/mL) for 1, 3 and 24 hours and the following parameters were evaluated: reactive oxygen and nitrogen species production; protein and lipid oxidation; superoxide dismutase (SOD) activity; reduced glutathione (GSH). IL-6 and IL-8 levels were also measured. One-way ANOVA test and Dunnett?s test as post hoc test were used for statistical analysis.Results: The cells exposed to 50 and 100 μg/mL of DEP showed a significant increase in reactive oxygen species and in reactive nitrogen species production compared to the control group, as well as SOD activity at all time points evaluated. DEP 50 and DEP 100 groups also presented an increase in lipid oxidation after 1 hour of incubation in accordance to the maximum (rised by) of reactive oxygen species production. Protein oxidation was found increased in DEP 100 groups only after 24 hours (58%, p