Oxidative and inflammatory response of RAW 264.7 murine macrophages exposed to air pollution particulate matter

GARCÉS, MARIANA; GONZÁLEZ MAGLIO, DANIEL; PAZ, MARIELA; CÁCERES, LOURDES; TASAT, DEBORAH; OJEDA, DIEGO; EVELSON, PABLO; MARCHINI, TIMOTEO

Buenos Aires

Congreso; III International Congress in Translational Medicine; 2016

Universidad de Buenos Aires

The exposure to environmental particulate matter (PM) is associated with increased cardiorespiratory morbidity and mortality rates. Alveolar macrophages are suggested to play a central role in this scenario, triggering both inflammation and oxidative stress following PM inhalation. However, the pathways linking these responses remain uncertain. In order to address such mechanisms, we studied the murine macrophage cell line RAW 264.7 after an exposure to different PM samples, Residual Oil Fly Ash (ROFA) and Concentrated Ambient Particles (CAPs), at 0, 50, and 100 μg/mL for 24 h. Intracellular redox status was assessed by dichlorofluorescein fluorescence, which was increased in a dose-dependent manner by up to 21% and 46% at 100 μg/mL of ROFA and CAPs, respectively (control: 160 ± 5 (x102) AU, p