IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of mitochondria and energy metabolism in low, intermediate and severe endotoxemia
Autor/es:
VICO, TAMARA; VANASCO, VIRGINIA; EVELSON, PABLO; ALVAREZ, SILVIA
Lugar:
Montevideo
Reunión:
Simposio; Protein Oxidation and Turnover: relevance in Biology and Medicine; 2016
Institución organizadora:
CEINBIO
Resumen:
It is known that endotoxemia is associated to mitochondrial dysfunction and oxidative stress. Low-grade endotoxemia is related to aging, cardiovascular, metabolic and neurological diseases whereas acute endotoxemia is associated to systemic inflammatory syndromes such as sepsis. The aim of this study is to analyze mitochondrial function in a rat model of low, intermediate and severe endotoxemia. Female Sprague-Dawley rats, 45 ± 5 days of age, were used and separated in five experimental groups. LPS was injected i.p. in a single dose of 0.5, 2, 4 and 8 mg/kg for each group, and control animals were injected with saline solution. After 6 h of treatment, animals were anesthetized and sacrificed, and the heart was rapidly excised. Mitochondria were isolated from left ventricle by differential centrifugation, and O2 uptake and respiratory chain complex activity were measured. O2 uptake in state 4 (using malate and glutamate as substrates) and state 3 (with the addition of ADP) was measured. Although the values were not statistically significant, a decrease in state 3 respiration associated to endotoxemia severity was observed. This result was accompanied with decreased respiratory control ratio (Control: 4.75; LPS 8 mg/kg: 4.29). Furthermore, NADH-cytochrome c reductase (Complex I/III) activity showed a statistically significant decrease at 4 mg/kg LPS doses (Control: 541 ± 96; 4 mg/kg LPS: 384 ± 96 nmol min-1mg prot-1; p