IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lung O2 metabolism after an acute exposure to transition metals present in nanoparticles.
Autor/es:
MARCHINI, TIMOTEO; MAGNANI, NATALIA; GARCÉS, MARIANA; CACERES, LOURDES; MEBERT, ANDREA; DESIMONE, MARTIN; DIAZ, LUIS; ALVAREZ, SILVIA; EVELSON, PABLO
Lugar:
Ciudad de Buenos Aires
Reunión:
Congreso; Society of Environmental Toxicology and Chemistry Latin America 11th Biennial Meeting.; 2015
Institución organizadora:
Society of Environmental Toxicology and Chemistry Latin America
Resumen:
Several studies have shown that the exposure to commercial or therapeutic nanoparticles (NP) might trigger, under certain conditions, toxic effects over the respiratory system. It is suggested that transition metals present in NP could play an important role in this scenario, via increased production of reactive O2 species and oxidative tissue damage, through Fenton-like chemical reactions. To address this hypothesis, the aim of this work was to study lung O2 metabolism after an acute exposure to transition metal-doped nanoparticles (NP). NP were built by the Stöber method. Their aerodynamic diameter was assessed by dynamic light scattering and metal content by atomic absorption. Female Swiss mice (25 g) were intranasally instilled with a suspension of silica NP containing Ni (II), Cd (II), Fe (III), or Cr (VI); at 0.01, 0.05, 0.1, and 1.0 mg metal/kg body weight. Control mice were handled in parallel and exposed to silica NP without transition metals. Tissue O2 consumption, NADPH oxidase (Nox) activity, and TBARS content were evaluated in lung samples 1 h after instillation. No changes were observed after Cd-NP exposure in any experimental condition. On the contrary, Ni-NP showed a significant increase by 35% to 67% in lung O2 consumption at every tested concentration when compared to the control group (p