IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lung O2 metabolism after an acute exposure to transition metals present in ambient particulate matter
Autor/es:
GARCÉS, MARIANA; MAGNANI, NATALIA; MARCHINI, TIMOTEO; CACERES, LOURDES; MEBERT, ANDREA; DESIMONE, MARTIN; DIAZ, LUIS; ALVAREZ, SILVIA; EVELSON, PABLO
Lugar:
Ferrara
Reunión:
Congreso; Cellular Environmental Stressors in Biology and Medicine; 2014
Institución organizadora:
Universita di Ferrara
Resumen:
Transition metals could play an important role in lung diseases mechanisms triggered by the exposure to ambient particulate matter (PM). The aim of this work was to study lung O2 metabolism after the exposure to transition metals-doped nanoparticles (NP). Swiss mice were intranasally instilled with silica NP containing Cd, Fe or Cr (0.01; 0.05; 0.10; 1.0 mg metal/kg body weight). NP were built by Stöber method, their aerodynamic diameter was assessed by dynamic light scattering and the metal content by atomic absorption. Tissue O2 consumption, NADPH oxidase (Nox) activity and TBARS content were evaluated in lung samples 1 h after instillation. No changes were observed after Cd-NP exposure in any dose or parameter evaluated. Fe-NP showed significantly increased O2 consumption, Nox activity and TBARS content at higher doses (0.01 to 1.0 mg Fe/kg body weight). Regarding Cr-NP exposure, tissue O2 consumption and TBARS levels were significantly increased at 0.05 mg Cr/kg body weight; Nox activity showed no significant changes. Transition metals like Fe and Cr seem to be important components involved in the altered lung oxidative metabolism after PM exposure. These findings provide new insights to the understanding of lung oxidative damage mechanisms triggered by metals present in environmental PM.