IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Air particulate matter: another emergency for skin health
Autor/es:
MAGNANI, NATALIA; MURESAN, XIMENA; BELMONTE, GIUSSEPPE; CERVELLATI, FRANCO; STICOZZI, CALAUDIA; MARCHINI, TIMOTEO; EVELSON, PABLO; VALACCHI, GIUSEPPE
Lugar:
Andros
Reunión:
Congreso; 6th International Conference on Oxidative Stress in Skin Medicine and Biology; 2014
Institución organizadora:
Oxygen Club of California
Resumen:
Epidemiologic studies have suggested a correlation between increased air particle matter (PM) concentrations and adverse health effects. Humans enter in contact with different air pollutants via inhalation, ingestion and dermal contact. Cutaneous tissue is continuously exposed to outdoor stressors such as air pollutants. The mechanisms of PM-health effects are believed to involve oxidative stress and inflammation. Air pollution is comprised by a wide range of chemicals and solid particles. The oxidative capacity of PM is primarily attributed to its transition metal constituents, which typically include Fe, V, Cr, Mn, Co, Ni, Cu, Zn, and Ti. Some of these metals can catalyze Fenton-type reactions and generate reactive O2 species, which are able to initiate oxidative damage mechanisms. Moreover, through semi-quinone, lipopolysaccaride, hydrocarbon, and ultrafine constituents, PM may also exert oxidative stress by presenting or by stimulating the cells to produce reactive O2 species. To evaluate the ability of PM to promote oxidative stress and tissue damage, we studied a skin tissue exposure to concentrated ambient particles (CAPs). Reconstructed human epidermis (RHE) was exposed to 25 µg/mL or 100 µg/mL CAPs for 24 or 48 hs. LDH released, HNE content were evaluated in all tissue samples after the exposure. Taking into account the LDH released values obtained after, we observed that RHE seems to be more susceptible to CAPs induced toxicity after 48 hs exposure regardless the particles dose. In addition, electromicroscopy was able to show the ability of the particles to penetrate skin tissues. A local reactive O2 species production increase could be generated from chemicals coated on the particle surface, which, in turn, may lead to macromolecules, such as lipids, oxidative damage. These findings contribute to the understanding of the skin pathophysiological mechanisms initiated by CAPs exposure where oxidative stress may play a predominant role. Oxidative stress appears to be an important area of study in skin damage, triggered by PM exposure, in order to clarifying the molecular mechanisms involved.