IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Dopamine effects on striatal mitochondrial function.
Autor/es:
CZERNICZYNIEC, A.; BUSTAMANTE, J.; LORES-ARNAIZ, S.
Lugar:
Varadero, Cuba
Reunión:
Workshop; 1st International Workshop of Neuroimmunology.; 2008
Institución organizadora:
Cuban Society of Pharmacology
Resumen:
Dopamine (DA) is able to induce neurotoxic effects in several neurological and psychiatrics diseases. It has been postulated that dopamine may generate changes on mitochondrial function by inhibition of respiratory chain. The aim of this work was to determine the in vitro effects of dopamine on striatal mitochondrial function. Dopamine (DA) and oxidation products have been related to mitochondrial dysfunction. Striatal submitochondrial membranes and intact mitochondria and were incubated with different DA concentrations for 5 minutes. Significant changes were observed in state 4 oxygen uptake (resting respiration) after 1 mM dopamine incubation. A 35% decrease in state 3 oxygen uptake (active respiration state) was found after 1 mM dopamine incubation. Incubation of submitochondrial membranes with 0.5-0.75–1 mM dopamine inhibited cytochrome oxidase by 38%, 46% and 53% respectively showing the possible inhibition or mitochondrial respiration through the inhibition of cytochrome oxidase. Complex I activity was significantly inhibited by incubation of striatal submitocohondrial membranes with 1 mM dopamine. Hydrogen peroxide production significantly increased by 91% 1 mM DA incubation. Also, DA was able to induce striatal mitochondrial membrane depolarization. Our results suggest that in our study conditions, DA modifies striatal mitochondrial function trhough inhibition of oxygen consumption, inhibition of cytochrome oxidase activity, increase in hydrogen peroxide production and mitochondrial membrane depolarization.