Oxidative stress markers in visual cortex and red blood cells of a glaucoma model.

REIDES, CG; LERNER, F; LASAGNI VITAR, RM; FERREIRA, SM; LLESUY, SF

CABA

Congreso; VIII Meeting of the SFRBM; 2013

South American Group

The aim of the present study was to establish the antioxidant status in visual cortex homogenates and red blood cells (RBC) of an experimental glaucoma model. Wistar rats (n= 5) weighing 250-300 g were operated under a microscope by cauterized two of the episcleral veins (GG) and another sham procedure group (n= 5) (CG). In order to assess the occurrence of oxidative stress the following markers were evaluated at 45 days after the surgery in right (RVC) and left visual cortex (LVC): spontaneous chemiluminescence(CL), TBARS, total antioxidant capacity(TRAP), thioredoxin reductase (TR) and glutathione peroxidase (GPx). In RBC superoxide dismutase (SOD), catalase (CAT) and total glutathione (GSH+GSSG) were measured. A significant increase of 78%(RVC) and 75%(LVC) in TBARS and of 104%(RVC) and 89%(LVC) in CL, a decrease in TR activity of 54%(RVC) and 34 %(LVC) and an increase in GPX activity of 71%(RVC) and 51%(LVC) were found in GG compared to CG. RBC of GG showed a significant increase of 48% in SOD. Reactive oxygen species are increased in glaucoma evidenced by an increase in TBARS and CL values. The decrease in TR and a compensatory up-regulation of SOD and GPx may be a consequence of an increase in oxidative process.