kidney mitochondrial function in experimental endotoxemia: effect of a-lipoic acid

CIMOLAI MARÍA CECILIA; VIRGINIA VANASCO; MARCHINI TIMOTEO; MAGNANI NATALIA D.; EVELSON PABLO; ALVAREZ SILVIA

Buenos Aires

Congreso; VIII International Congress of SFRBM South American Group.; 2013

Society for Free Radical Biology and Medicine

The aim of this work was to evaluate the beneficial effects of LA in kidney bioenergetics during endotoxemia, emphasizing the importance of an adequate antioxidant status for maintaining mitochondrial function in pathology. In rats (Sprague-Dawley, female, 45 ± 5 days old) treated with lipopolysaccharide (LPS, 10 mg/kg), no differences were observed in mitochondrial O2 consumption, mitochondrial complex II activity, and ATP production. Pretreatment with α-lipoic acid prevented the 1.2 fold increase in NO production, decreased (30-40%) mitochondrial complex I and IV activities, decreased (26%) membrane potential and cardiolipin oxidation (76%) observed in LPS-treated animals. This work provides new evidence that the in vivo treatment with α-lipoic acid is a therapeutic strategy and/or antioxidant supplementation that may be considered for the septic syndrome, based on the improvement of the mitochondrial function, decreased production of mitochondrial NO and restoration of cardiolipin levels.